Background: Insecticide-treated nets (ITNs) combining pyrethroids with piperonyl butoxide (PBO) have improved the control of malaria transmitted by pyrethroid-resistant vectors compared to standard pyrethroid-only ITNs. To sustain malaria prevention efforts, a broader range of effective pyrethroid-PBO nets is needed to enhance market diversity and supply resilience. This study evaluated the entomological efficacy and wash durability of Vector Guard®, a new mosaic alpha-cypermethrin-PBO ITN, against pyrethroid-resistant Anopheles gambiae s.l. in southern Benin.